Cell type . This MAb is reportedly useful for identifying tumors arising from smooth muscle and myoepithelial cells. To understand whether actin isoforms have specific functions during cardiac . The main proteins in thin filaments of smooth muscle are actin, Tm, caldesmon (h-CaD), and calmodulin, but no troponin (Smith and Marston, 1985). The smooth muscle -actin and -actin proteins are found primarily in vascular smooth muscle and enteric smooth muscle, respectively. Cancer-associated fibroblasts (CAFs) demonstrate the characteristics of myofibroblast differentiation by often expressing the ultrastructure of alpha-smooth muscle actin (SMA). Anti-soluble liver antigen/liver pancreas (anti-SLA/LP) antibodies. However, the function of peri-implantation uterine smooth muscle and the regulation mechanism of muscle tension are still unclear. These include: Antinuclear antibodies. This actin is an alpha actin that is found in skeletal muscle. Smooth muscle actin reacts with the alpha-smooth muscle isoform of actin, and labels the smooth muscle cells of vessels, myoepithelial cells, pericytes, some stromal cells in the intestine, testis, and ovary, and tumors derived from smooth muscle cells. Specifications Specifications Datasheets & SDS Phalloidin is a highly selective bicyclic peptide used for staining actin filaments (also known as F-actin). However, its role in hepatocellular carcinoma (HCC) is less clear. Alpha-smooth muscle actin (-SMA) was examined immunohistochemically in intratumoral CAFs of nonmetastatic breast cancers and correlated with clinicopathological data. Alpha Smooth Muscle Actin (SMA) Immunohistochemistry Use in the Differentiation of Pancreatic Cancer from Chronic Pancreatitis Authors Katarzyna Winter 1 , Monika Dzieniecka 2 , Janusz Strzelczyk 3 , Magorzata Wgrowska-Danilewicz 4 , Marian Danilewicz 4 , Ewa Maecka-Wojciesko 1 5 Affiliations Mouse monoclonal anti-actin, -smooth muscle antibody has been used for western blot assays using whole kidney homogenates in human anaplastic thyroid cancer cells. Actin (Smooth Muscle) Antibody (IgG) - Actin is the major antigen to which smooth muscle antibodies react in autoimmune hepatitis. Smooth muscle tissue is found in many areas of the body, such as the digestive system, urinary system, blood vessels and uterus. ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] IHC-P image ab5694 reacts with tumors arising from smooth muscles and myoepithelial cells. An autoantibody attacks the body's own cells and tissues by mistake. This study focused on the molecular mechanism of actin assembly regulation on implantation in smooth muscle. When a muscle contracts, the actin is pulled along . Smooth muscle antibodies (SMA) are autoantibodies, proteins produced by the body's immune system that recognize and attack its own actin, a protein found in smooth muscle and other tissues, especially the liver. Product Specific Information Calculated MW 42 kDa. However,. Smooth muscle is composed of sheets or strands of smooth muscle cells. 25, 26 thus, they may obtain new features that are not present in The aim of present study was to investigate the prognostic and functional significance of ACTA2 expression in cancer cells for the metastatic potential of lung adenocarcinomas. An anti-smooth muscle antibody (ASMA) test is a blood test that detects autoantibodies that attack the smooth muscle cells in the body, often in the liver. Your immune. Studies have indicated a significant correlation between a CAF-rich stroma and adverse survival prognostic factors in many human cancers, such as breast cancer and squamous cell carcinoma (SCC)3,15). Defects in this gene cause aortic aneurysm familial thoracic type 6. Methods: In total, 74 surgically resected gastric carcinomas (24 diffuse type, 43 intestinal type, and seven solid . Anti-actin antibodies. It starts as a growth of abnormal cells and often grows quickly . ACTA2 is the isoform of contractile protein alpha-actin present in vascular smooth muscle cells (SMCs) throughout the arterial tree.4 Pathologic conditions in individuals with ACTA2 mutations show increased deposition of SMCs in the intimal arterial layer, leading to a decreased intraluminal diameter.4 Leiomyosarcoma most often begins in the abdomen or uterus. Smooth muscle tumors are strongly positive for smooth muscle actin (SMA). for CAFs is -smooth muscle actin (-SMA), which is also a specific marker for myofibroblasts23). A newly identified mechanism of smooth muscle relaxation is the interaction between the small heat shock protein 20 (HSP20) and 14-3-3 proteins. 9-10 Anatomy And Physiology Exam 5 Human Anatomy Chapter 10 Physio Ch12 Chapter 9 Muscle A&P CH.9 Muscles And Muscle Tissue Chapter 12 MASTERING A&P Chapters 6,8,9,11 Phys Ch 12 A&P Chapter 9 Questions Chapter 9: Muscles And Muscle Tissue A&P Ch 8 Muscles LS 7C . Antibody staining with HPA003180 in immunohistochemistry. The product has also been used for immunohistochemical assays using mice tissue sections in liver sections and uterine sections. This may be taken through a vein. Within single-unit muscle, the whole bundle or sheet of smooth muscle cells contracts as a syncytium.. smooth muscle actin. Smooth muscle actin ( -SMA) and stroma evaluation A total of 183 formalin-fixed paraffin-embedded tissue samples could be collected retrospectively. MRC-5 fibroblasts are one of the CAFs expressing alpha-smooth muscle actin. F-Actin IGG antibodies are found in 52-85% of patients with Autoimmune Hepatitis (AIH) or chronic active hepatitis and in 22% of patients with Primary Biliary Cirrhosis (PBC). $ 469.95 Products Downloads Products SMA-L-CE 1ml NCL-L-SMA In Stock Ships via Overnight Online-only price (details) $ 469.95 What is an anti-smooth muscle antibody (ASMA) test? BIOCARE's 1A4 MAb stains smooth muscle cells in vessel walls, gut wall, and myometrium. 104 Sarcomeric actin is expressed in skeletal muscle tumors and to a lesser extent in smooth muscle tumors, but its use in clinical practice is limited because of low sensitivity and specificity. 101 It is divided into two subgroups, single-unit and multiunit smooth muscle. The smooth muscles are in the hollow organs of the body, including the intestines, stomach, bladder, and blood vessels. CAFs play important roles in tumor growth. -Smooth muscle actin is first and transiently expressed, followed by -skeletal and finally -cardiac actin. It is difficult to obtain a definitive diagnosis for nodular smooth muscle proliferation (NSMP) before surgery, and a pathological diagnosis is necessary to differentiate it from primary lung cancer. SMAs attack smooth muscle tissues in the liver and other parts of the body. Toll-like receptor 9 expression has been observed in different type of cancer. All smooth muscle tumors, and particularly epithelioid leiomyosarcomas, may have patchy cytokeratin positivity. Cancer microenvironment is a complex of different kinds of cells. It is ascertained that medium conditioned by MRC-5 fibroblasts stimulate motility and invasion of breast cancer cells. Product ID: smooth-muscle-actin-alpha-sma Antigen Background Cytoplasmic actins are part of the microfilament system of cytoskeletal proteins. Source Monoclonal Mouse IgG 2A Clone # 1A4 Purification Protein A or G purified from hybridoma culture supernatant Immunogen alpha -Smooth Muscle Actin N-terminal Synthetic Decapeptide Formulation Supplied in a saline solution containing BSA and Sodium Azide. KHC0053 is a ready-to-use IHC kit for staining of smooth muscle actin (ASMA). Aims: To investigate the distribution and roles of smooth muscle actin (ASMA) positive stromal cells (ASMA+ cells), which belong to the myofibroblast group, within gastric carcinomas, with reference to three histological types (diffuse type, intestinal type, and solid type). The kit provides all reagents, from antigen retrieval to cover slip mounting, that require little to no diluting or handling prior to use. Evaluation of stromal density and -SMA. In females, there is also smooth muscle in the uterus. These actin isoforms regulate the contractile potential of muscle cells (1). Smooth muscle alpha actin is one of a few genes whose expression is predominantly expressed in vascular smooth muscle cells that is used to study myofibroblasts and fibrosis (1). Myoepithelial cells in breast and salivary gland are also stained. Defects in the smooth muscle actin gene cause aortic aneurysm familial thoracic type 6. Volume 2 (2022) - Issue 3; Volume 2 (2022) - Issue 2; . Smooth muscle alpha-actin (SMA) is the definitive marker for the activated cardiac fibroblast population known as the myofibroblast ( Frangogiannis et al., 2000 ), but this actin isoform is also abundant in vascular smooth muscle cells ( Skalli et al., 1989 ). Specificity. Smooth muscle is a type of muscle tissue which is used by various systems to apply pressure to vessels and organs. These cells have fibers of actin and myosin which run through the cell and are supported by a framework of other proteins. Download Citation | Molecular Mechanism of Mouse Uterine Smooth Muscle Regulation on Embryo Implantation | Myometrium plays critical roles in multiple processes such as embryo spacing through . This test requires a blood sample. Home; smooth muscle actin; By admin; 0 Comments; Tumour-associated Angiogenesis and Intermediate Blood Vessels in Renal Cell Carcinoma. Leiomyosarcoma, or LMS, is a type of rare cancer that grows in the smooth muscles. -Smooth muscle actin (-SMA) is an indicator of myofibroblast. Expression of TNRC6B (KIAA1093) in smooth muscle tissue. An anti-smooth muscle antibody (ASMA) test detects antibodies that attack smooth muscle. The procedure is called a venipuncture. The cells were then stained with ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] (red) at 1:100 dilution in 1x PBS/1% BSA for 30 min at 4C, followed by Alexa Fluor 488 labelled secondary antibody. Description: Actin Smooth Muscle Antibody Blood Test (Labcorp) This test measures the level of Actin Smooth Muscle Antibodies (ASMA) in the blood. The abnormal induction of -SMA expression was found to be associated with shorter disease-free survival in lung, colorectal and pancreatic cancers ( 4 - 6 ). Beta and gamma actin are found in muscle and non-muscle cells. Alpha actin is found in muscle cells and there are specific alpha actins for smooth, skeletal and cardiac muscle. Cancer Genomics & Proteomics; Contact; Home; Editorial Board; Current Issue; Archives. Four CAF cell lines were isolated from patients with luminal breast cancer (lumBC) and classified according to the presence of -SMA protein. SMA (Alpha Smooth Muscle Actin) is recommended for the detection of specific antigens of interest in normal and neoplastic tissues, as an adjunct to conventional histopathology using non-immunologic histochemical stains. How the Test is Performed A blood sample is needed. Smooth muscle antibodies are produced in people with certain types of autoimmune disorders.These disorders cause the body's immune system to produce antibodies that attack its own cells and tissues. Not tested in other applications. The diagnosis and management of autoimmune hepatitis may . ( Note ) Positive Control People with autoimmune hepatitis often have other autoantibodies. Mouse monoclonal [ACTA2/791] to alpha smooth muscle Actin - BSA and Azide free. . We report two cases of NSMP that were suspected to be primary lung cancer on preoperative images. Anti-Actin antibodies have been reported in 3-18% of sera from normal healthy controls. Actin gamma 2, smooth muscle: MYLK: Myosin light chain kinase: ACTC1: Actin alpha cardiac muscle 1: AIF1: Allograft inflammatory factor 1: MYH11: Myosin heavy chain 11: ACTN2: Actinin alpha 2: ACTN3: Actinin alpha 3: ACTN1: Actinin alpha 1: Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. Smooth muscle actin is found on smooth muscle vessel walls, gut wall, myometrium, myoepithelial cells in breast and salivary glands. Myoepithelial cells in breast and salivary glands are also stained as they also contain actin. The alpha isoform of smooth muscle actin (SMA) is known to be expressed in smooth muscle cells, myoepithelial cells, pericytes and myofibroblasts [ 3, 4 ]. The smooth muscle (SMA) or actin antibody test is primarily ordered along with antinuclear antibodies (ANA) and liver kidney microsomal type 1 (LKM-1) antibodies to help diagnose autoimmune hepatitis and to differentiate between the two major types of autoimmune hepatitis, type 1 and type 2. Leiomyosarcoma is a rare type of cancer that begins in smooth muscle tissue. Application: IHC-P. Reactivity: Rat, Human (predicted: Mouse, Rabbit, Chicken, Cow) Conjugate: Unconjugated. Smooth muscle or "involuntary muscle" consists of spindle shaped muscle cells found within the walls of organs and structures such as the esophagus, stomach, intestines, bronchi, uterus, ureters, bladder, and blood vessels. Leiomyosarcoma. Cancer Cardiovascular Cell Biology Epigenetics Metabolism Developmental Biology By research area Immunology Microbiology Neuroscience Signal Transduction . Sun KH, Chang Y, Reed NI, Sheppard D. -Smooth muscle actin is an inconsistent marker of fibroblasts responsible for force-dependent TGF activation or collagen production across multiple models of organ fibrosis. myofibroblasts are different from normal fibroblasts in many aspects, including (1) rued membranes and a highly active endoplasmic reticulum ( baum and duffy, 2011 ); (2) expression of alpha smooth muscle actin (-sma or acta2) and increased levels of vimentin (vim) ( ronnov-jessen and petersen, 1993) and (3) formation of complex and organized Function [ edit] This gene is an ortholog of the Drosophila fat gene, which encodes a tumor suppressor essential for controlling cell proliferation during Drosophila development. Smooth muscle actin is found in vascular walls, intestinal muscularis mucosae and muscularis propria and in the stroma of various tissues. By DSP, high expression of alpha-smooth muscle actin (-SMA), both in the leukocyte and stromal compartments, was associated with shorter DFS in univariate analysis (P = 0.002 and P = 0.023, respectively).High -SMA expression in the stroma was validated by QIF after controlling for estrogen receptor and progesterone receptor status [HR, 3.12; 95% confidence interval (CI), 1.12-8.68; P = 0 . GTX100034 Reference 75 7 Datasheet File See all alpha Smooth Muscle Actin products APPLICATION Application Note *Optimal dilutions/concentrations should be determined by the researcher. A smooth muscle antibody (SMA) is a type of antibody known as an autoantibody. Unlabelled sample (green) was used as a control. Simply apply the reagents to your sample slide according to the protocol and you're steps away from obtaining high-quality IHC data. Ligands of different Toll-like receptors has been used in clinical trials in past decade but the mixed outcome leads to further studies.
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