Board review style question #1. 2F7C & XH13Z3 - neoplasm of uncertain behavior of connective or other soft tissue & desmoid type (aggressive) fibromatosis. Twenty-nine of 33 cases of epithelioid fibrous histiocytoma (88%) showed diffuse cytoplasmic staining for ALK ( Figures 1 and 2 . Because of the plethora of diagnoses and often subtle nature of diagnostic criteria, IHC finds particular utility in soft tissue tumors. Calretinin, a routine antibody used in surgical pathology practice, has been described elsewhere . On DermNet NZ It is also expressed in neoplasms with smooth or skeletal muscle differentiation. Although there has been a considerable progress in automation and . Sections of formalin-fixed, paraffin-embedded canine leiomyomas, leiomyosarcomas, or fibrosar- comas were examined by immunohistochemical methods for the presence of desmin. Peckham, A. Immunohistochemistry. (B) Abdominal desmoid tumour showing the typical infiltrative growth pattern of the skeletal muscle (haematoxylin and eosin staining; original magnification, 200). Immunohistochemistry (IHC) is an important auxiliary method for pathologists in routine diagnostic work as well as in basic and clinical research including exploration of biomarkers, as IHC allows confirmation of target molecule expressions in the context of microenvironment. The labeling is confined to the Z bands in skeletal and cardiac muscle giving a characteristic striated appearance. This test helps your pathologist decide if the tumour is made up of muscle cells or if the cells are starting to behave like muscle cells. In neoplastic tissues, the antibody reacts with tumors of myogenic origin such as those arising from smooth muscle (leiomyosarcomas) and those derived from striated muscle (rhabdomyosarcomas). Catalog Number: Size: Price: Availability: IW-MA1036: 9 ml: $258: 1-2 weeks: Description: Desmin belongs to the type III family of intermediate filaments, a class of cytoskeletal elements. Ready To Use . Supplier: Dako. In brief, sections . However, in some examples, desmin immunohistochemistry can demonstrate a nesting pattern/lobulated arrangement. IHC was carried out using the Envision Plus/Horseradish Peroxidase system (Dako, Carpinteria, CA), a polyclonal antibody to PAX8 (Proteintech Group Inc, Chicago, IL, 1:800 dilution), all cases stained at a dilution of 1:300. As a relatively uncommon neoplasm and locally aggressive cutaneous tumor, it is characterized by high rates of local recurrence, but a low risk of metastasis ( 1 - 4 ). Desmin is recommended for the detection of specific antigens of interest in normal and neoplastic tissues, as an adjunct to conventional histopathology using non-immunologic histochemical stains. Desmin positivity (usually focal) was only sporadically encountered in the benign gastric and small intestinal GISTs (5% and 3%) and was never seen in the malignant GISTs of stomach and small. Diagnostic Criteria; Multiple/Hereditary . Determining the cytokeratin (CK) 7/CK20 pattern of tumors is one of the most helpful procedures for this purpose since the CK7-/CK20+ pattern is typical of colorectal adenocarcinomas. Add to Quote VIEW DOCUMENTS Endometrial Stromal Sarcoma (Metastatic) . Histopathology of desmoid tumours and control samples. Tumor cells revealing diffuse immunoreactivity to smooth muscle actin (IHC for smooth muscle actin 400x). Products Downloads Products The antibody labels smooth and striated muscle cells as well as mesothelial cells, and is a useful aid for the classification of rhabdomyosarcoma (1, 2), leiomyoma (3, 4), Am J Pathol 1985;118:85, Hum Pathol 1985;16:838, Am J Clin Pathol 1991;96:32. Download Stains by CPT Code 88342 88342:(Global Only) 88312 88313 88365 (in-situ hybridization) Double-Stains Triple-Stain Panels Immunogloblun G (IgG) 88342 Adenovirus Adrenocorticotropic hormone (ACTH) Alk-1 protein Alpha 1 antichymotrypsin/A1ACT Alpha [] Differential Diagnosis by Histologic Pattern View large Department of Veterinary Pathology, College of Veterinary Medicine, University of Georgia, Athens, GA Abstract. By immunohistochemistry the tumor cells are positive for desmin, h-caldesmon, ER and PR. Anti-Human Desmin Clone D33 Code M0760 Intended use For in vitro diagnostic use. Monoclonal Mouse Anti-Human Desmin, Clone D33, is intended for use in immunohistochemistry (IHC). Faculty of Biological Sciences, Leeds University, 2003. If the cells in your sample produce desmin, your pathology report will describe the cells as positive or reactive. calponin and smmhc are two antibodies that are more specific for mecs. Desmin Immunostain, Technical Component Only Useful For Identification of striated and smooth muscle cells and tumors derived from this cell type Reflex Tests Testing Algorithm For the initial technical component only immunohistochemical (IHC) stain performed, the appropriate bill-only test ID will be reflexed and charged (IHTOI). Clone: DE-R-11, Mouse anti-Porcine. DES gene encodes desmin, a muscle-specific cytoskeletal protein found in smooth, cardiac, and heart muscles. The findings support the above diagnosis. We included 120 surgically resected pancreatobiliary tract cancer cases59 cases as a test set with known VI and 61 cases as a validation set without information of VI. Mntrier disease and Cronkhite-Canada syndrome may also need to be ruled out. Diffuse desmin and focal myogenin positivity supports the diagnosis of botryoid embryonal rhabdomyosarcoma Additional references. Abstract. Surgical Pathology Criteria . Both are desmin positive with a prominent sclerotic/desmoplastic stroma Other sarcomas with a prominent sclerotic/desmoplastic background may also mimic sclerosing rhabdomyosarcoma These include, but are not limited to, osteosarcoma, extraskeletal myxoid chondrosarcoma, epithelioid fibrosarcoma and angiosarcoma ICD coding. This cellular tumor group exhibited consistent vimentin immunoreactivity, but desmin was negative or only weakly and/or focally positive. The pitfalls of studying desmin immunohistochemistry as a routine diagnostic tool in surgical pathology are discussed. Disclaimer Desmin is recommended for the detection of specific antigens of interest in normal and neoplastic tissues, as an adjunct to conventional histopathology using non-immunologic histochemical stains. Immunohistochemistry (IHC) plays an important role in the diagnosis of soft tissue tumors. 2F7C & XH6116 - neoplasm of uncertain behavior of connective or other soft tissue & abdominal (mesenteric) fibromatosis. 68,69 In smooth muscle cells, desmin is seen with cytoplasmic dense bodies and subplasmalemmal dense plaques; in striated muscle, desmin filaments are linked to sarcomeric Z disks. Desmin IHC Antibody . 24 calponin, a 34-kd polypeptide, modulates actomyosin adenosine triphosphatase (atpase) activity in the Central Coast Pathology Laboratory. Definition / general Actin is a 43000 kDa ubiquitous protein found in all cells Actins are involved in cell motility (alpha, smooth muscle) and the maintenance of the cytoskeleton (beta and gamma, all cells) Antibodies to alpha smooth muscle actin do not detect the other actin isoforms Essential features Involved in cell motility The most important differentiating feature of these conditions is their diffuse mucosal involvement rather than the formation of distinct polyps. . Stanford Medicine School of Medicine Departments Surgical Pathology Criteria GIST Gastrointestinal Stromal Tumor. Incubation Time/Temp: 60 minutes/room temperature Kiupel, M. Diagnostic molecular pathology. Pathologists perform a test called immunohistochemistry to look for cells producing desmin. . Sample pathology report. For additional technical details, contact ARUP Client Services at (800) 522-2787. Vagina, excision biopsy: . Desmin is recommended for the detection of specific antigens of interest in normal and neoplastic tissues, as an adjunct to conventional histopathology using non-immunologic histochemical stains. Interpretation Dilution: 1:30 using IHC-Tek TM Antibody Diluent (Cat# IW-1000 or IW-1001) to reduce background and unspecific staining and serum blocking step is NOT needed. 4 ), although some cases are nearly completely pleomorphic with only In addition to the many established IHC markers currently in use, new markers continue to emerge, although their general acceptance and routine application requires robust validation. Desmin, keratin and ALK negative : CD117, DOG1, CD34 negative : CD117 74-95%, DOG1 87-95%, CD34 70% . Caldesmon binds calcium, calmodulin, tropomyosin and actin and regulates smooth muscle contraction ( Int J Biochem Cell Biol 1997;29:1047 ) May play a role in preventing unwanted contractions ( Am J Physiol Heart Circ Physiol 2009;297:H1930 ) h-caldesmon is h igh molecular weight isoform, found in smooth muscle cells; l . Precut slides from paraffin blocks in 5 micron sections. 2015. We evaluated the patterns of VI and the added benefit provided by CD31, desmin, and dual CD31desmin immunolabeling for identification of VI. Twenty-two leiomyomas Smooth Muscle Skeletal Muscle Myofibroblasts (usually only express actin and vimentin, but may occasionally . Desmin is an intermediate filament that is expressed in both smooth and skeletal muscle myocytes. Definition / general Benign nerve sheath tumor arising from differentiated Schwann cells Essential features Encapsulated, well circumscribed Most cases have a zonal pattern composed of cellular areas (Antoni A) with nuclear palisading (Verocay bodies) and a hypocellular component (Antoni B) Terminology Neurilemoma (acceptable) ICD coding Catalog Number: M0724. ICD-10: D48.1 - neoplasm of uncertain behavior of connective and other soft tissue. 21-23 smmhc is a structural component (200 kd) unique to smooth muscle cells, which functions within the hexagonal array of the thick-thin filament contractile apparatus. 3. IHC positivity to desmin, myogenin, SMA, MyoD1 Spindle cell and sclerosing features Uniformly distributed tumor cells H&E, desmin, myogenin Cytology images Images hosted on other servers: Fine needle aspiration Positive stains Strongly positive for MyoD1 (diffuse) and vimentin (diffuse) Special stains and immunohistochemistry. S-100 protein was fundamentally negative in these tumors. Fibromatosis (also termed desmoid-type fibromatosis) of the breast is a rare neoplasm (<1% of breast lesions) composed of fibroblasts and myofibroblasts, arising either within the breast parenchyma or the pectoral fascia of the chest wall. If you do not have electronic ordering capability, use an ARUP Immunohistochemistry Stain Form (#32978) with an ARUP client number. Definition Keratin and desmin positive neoplasm composed of nests of small cells with surrounding desmoplastic stroma Alternate / historical names Desmoplastic small round cell tumor Intraabdominal desmoplastic small round cell tumor Small cell tumor with desmoplastic stroma Diagnostic criteria The results of ALK immunohistochemistry are summarized in Table 1. Citing Literature Volume38, Issue4 $ 392.95 Products Downloads Products Products DES-DERII-L-CE 1ml NCL-L-DES-DERII In Stock Ships via Overnight Online-only price (details) $ 392.95 Qty After ~30 years of widespread usage, immunohistochemistry (IHC) has become a standard method of diagnosis for surgical pathology. Cytoplasmic (because it is an intermediate filament, desmin often has a fibrillary staining quality). Immunohistochemically, the tumor cells are immunoreactive for keratin AE1 / AE3 and desmin and show strong nuclear expression for WT1 (C terminus), while all of the following are negative: myogenin, MYOD1, S100 and WT1 (N terminus). (A) Macroscopical view of the whitish cut surface of an extra-abdominal desmoid tumour. Products Downloads Products DES-DERII-L-CE 1ml NCL-L-DES-DERII Country availability Contact Us for a quote. Table 1. Methodology Immunohistochemistry (IHC) Specimen Requirements Information on collection, storage, and volume Specimen Formalin-fixed paraffin embedded (FFPE) tissue block with Corresponding H&E, and/or unstained slides sectioned from tissue block. Despite multimodal treatment, DSRCT is associated with dismal outcomes. Name: Desmin Antibody. Discussion Sclerosing stromal tumor is a benign subtype of ovarian sex cord stromal tumor, described as a distinct entity in 1973 by Chalvardjian and Scully [ 1 ]. David J. Dabbs MD, in Diagnostic Immunohistochemistry, 2019 Desmin. Desmin is a cytoplasmic IFP that is characteristically found in muscle cells and in neoplasms with myogenic differentiation. Clear cell papillary renal cell carcinoma is a recently recognized renal neoplasm, composed of an admixture of cystic, glandular, solid, and papillary components, all lined by cells with clear . The ultimate goal for a pathologist is to render a specific diagnosis that provides diagnostic, prognostic, and therapeutic information to guide patient care. Diagnostic Center for Population and Animal Health, College of Veterinary Medicine, Michigan State University, 2010. Storage/Transport Conditions Room temperature (use cold packs for transport of blocks during summer months) CPT Code(s) 88342 Requisition Dermatofibrosarcoma protuberans (DFSP) is a superficial, low-grade, locally aggressive, spindle, fibroblastic, neoplastic lesion. Immunohistochemistry (IHC) is an important adjunct in the diagnosis of neoplastic skin diseases. Immunohistochemistry has become an essential ancillary examination for the identification and classification of carcinomas of unknown primary site (CUPs). CDX2, a critical nuclear transcription factor for intestinal development, is expressed in intestinal epithelium and adenocarcinomas. Remarks. Volume 0.2 cm x 0.2 cm x 0.2 cm tissue. Definition / general. Over the last decade, the diagnostic accuracy of organ- or tumour-specific immunomarkers and the clinical validation of effective immunohistochemical panels has improved significantly. IMMUNOHISTOCHEMISTRY ORDERING AND SUBMISSION DETAILS: Submit electronic request. Test Details Skeletal and smooth muscle tumors Methodology Immunohistochemistry Performed Monday - Friday Turnaround 2 - 3 business days Specimen Requirements For Ventana IHC, sections should be on Plus(+) charged slides.
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