It is a glycoprotein showing a broad band in SDS-PAGE, and is synthesized by macrophages (average molecular weight 92-110,000 daltons, depending on the origin of macrophages). Recommended Dilutions. In Yuan's study, according to the fluorination of anhydride reaction, the low molecular weight of 1.8kDa PEI was used to prepare PEIF. Cross-linking mass spectrometry analysis of lysosome-enriched fractions. 1A), presumably due to less glycosylation. LAMP2-deficient hepatocytes show accumulation of early autophagic vacuoles, . LAMP2 may plays a role in tumor cell metastasis. Alternatively, are positive, CSF analysis will allow targeted mutational screening; how- molecular investigations can be done, guided either by results of phenyl-ever, one should keep in mind that phenylalanine (Phe) load can be posi- alanine loading test or clinical symptoms with caveats as noted above.1 tive in heterozygote carriers for . These results indicated that miR221 may regulate TGF1induced HSC activation through inhibiting autolysosome function by directly targeting LAMP2. This antibody induces apoptosis of human microvascular endothelium in vitro [PMID: 18836458]. Deletion of the Lamp2 gene in Cell Dev. Lysosome-associated membrane protein 1 and 2 (LAMP1 and LAMP2) are two abundant lysosomal membrane proteins (1,2). To this purpose, astrocytes generate a highly interconnected intercellular network allowing rapid exchange of ions and metabolites through gap junctions, adjoined channels composed of hexamers of connexin (Cx) proteins, mainly Cx43. CONJUGATE: Unconjugated TESTED APPLICATIONS: WB APPLICATION NOTE: Mature, functional LAMP2 is extensively glycosylated with a variety of different N-linked and O-linked oligosaccharides with a total molecular weight of about 100-130 kDa. Autophagy Project . Mature, functional LAMP2 is extensively glycosylated with a variety of different N linked and O linked oligosaccharides, with apparent molecular weight ranging from 45-130 kDa. CD107b, LAMP-2, LAMPB, LGP110. Biology Area Department of Molecular Medicine and Medical Biotechnologies, University of Naples Federico II, Via Sergio Pansini 5, 80131 Naples, Italy . mechanical properties. Here we examined the role in AMD of lysosome-associated membrane protein-2 (LAMP2), a glycoprotein that plays a critical role in lysosomal biogenesis and maturation of autophagosomes/ phagosomes. Anti-LAMP-2 Antibody (H4B4) is a mouse monoclonal IgG 1 LAMP-2 antibody, cited in 325 publications, provided at 200 g/ml raised against adherent spleen cells of human origin LAMP-2 Antibody (H4B4) is recommended for detection of LAMP-2 of human origin by WB, IP, IF, IHC (P) and FCM It results from mutations in the lysosomeassociated membrane protein 2 ( LAMP2) gene. Mucopolysaccharidosis type IIIA (MPS IIIA) is a lysosomal storage disorder caused by N-sulfoglucosamine sulfohydrolase (SGSH) deficiency. Analyzing LAMP2 across placental mammals, our phylogenetic-based maximum likelihood analyses indicate that the majority of the coding sites undergo purifying selection. Antigen Molecular Weight: Predicted: 43 kDa; Apparent: around 110 kDa. Some researchers think the LAMP-2 protein may help . enzyme binding . Functional alterations of Cxs and gap junctions . In contrast, significantly less LAMP2 was observed in the retina, displaying a slightly lower molecular weight (Fig. LAMP2 was not precipitated using mouse serum IgG antibodies, lane 2. . Detects a band of approximately 105 kDa (predicted molecular weight: 100 kDa). Table of Atomic Elements and Weights Atomic Number Element Name Atomic Symbol Atomic Weight (g/mol) 1 Hydrogen H 1.00794 2 Helium He 4.002602 3 Lithium Li 6.941 4 Beryllium Be 9.012182 5 Boron B 10.811 6 Carbon C 12.0107 7 Nitrogen N 14.0067 8 Oxygen O 15.9994 9 Fluorine F 18.9984032 10 Neon Ne 20.1797 11 Sodium Na 22.98976928 12 Magnesium Mg Most Asn (N)-linked glycosylations are high-molecular-weight complex poly-N-acetyl-lactosamine (LacNAc) moieties which can protect the membrane from degradation by lysosomal hydrolases 7. lysosomal-associated membrane protein 2. Her2-lamp2 fusion protein promotes targeted cellular uptake through EGFR receptor-mediated endocytosis in HCT-116 cells. In some aspects, the AAV is in the lumen of the extracellular vesicle. Roifman syndrome is a rare inherited disorder characterized by spondyloepiphyseal dysplasia, growth retardation, cognitive delay, hypogammaglobulinemia, and, in some patients, thrombocytopenia. This glycoprotein provides selectins with carbohydrate ligands. platelet activation ; platelet degranulation ; protein stabilization . PE/ATTO 594 is a tandem conjugate, where PE is excited at 535 nm and transfers energy to ATTO 594 via FRET (fluorescence resonance energy transfer), which emits at 627 nm. LAMP2 is considered to constitute the rate limiting step in CMA . 1. LAMP 2 has a structure comprised of a large amino-terminal intra-lysosomal domain, a hydrophobic trans-membrane domain, and a short carboxyl-terminal cytoplasmic tail. In some aspects, the AAV is associated with the luminal surface of the extracellular vesicle. 5-FU and Exo were mixed by electroporation, . Lamp2, and Pink1 gene expression (Figure 4F). 34 (2):108610. The parameters have been computed for the following feature FT CHAIN 29-410 Lysosome-associated membrane glycoprotein This LAMP2 monoclonal antibody is generated from mouse immunized with LAMP2 recombinant protein. Tubulin Antibody (TU-02) is a mouse monoclonal IgM , cited in 1,392 publications, provided at 200 g/ml raised against amino acids 1-451 representing full length Tubulin of porcine origin Anti-alpha Tubulin Antibody (TU-02) is recommended for detection of Tubulin of mouse, rat, human and porcine origin by WB, IP, IF, IHC (P), FCM and ELISA Plays an important role in chaperone-mediated autophagy, a process that mediates lysosomal degradation of proteins in response to various stresses and as part of the normal turnover of proteins with a long biological half-live (PubMed:8662539, PubMed:11082038, PubMed:18644871, PubMed:24880125, PubMed:27628032). The human LAMP1gene is located on the long arm (q) of chromosome 13 at region 3, band 4 (13q34). The molecular mass, often called the molecular weight (MW), is the weight of all atoms in a given molecular formula. All lanes : Anti-LAMP2 antibody [H4B4] - Lysosome Marker (ab25631) at 1/500 dilution Lane 1 : HEK293 cell lysate at 30 g Lane 2 : HepG2 at 30 g LAMP2 is a marker of lysosmes and late endosomes. Gene Ontology Molecular Function. Removal of N-linked glycans from Lamp1/2 causes their rapid proteolysis 8. Mature, functional LAMP2 is extensively glycosylated with a variety of different N-linked and O-linked oligosaccharides with a total molecular weight of about 100-130 kDa. View Cart. Cells in a different medium are placed on glass coverslips in six-well dishes and cultured to 60-80% confluence. Both are transmembrane proteins and are heavily glycosylated at the amino-terminal luminal side of the lysosomal inner leaflet, which protects the proteins from proteolysis (3). Medical and device therapies that reduce morbidity and mortality in patients with heart failure (HF) with a reduced left ventricular (LV) ejection fraction also lead to decreased LV volume and mass, and a more normal elliptical shape of the ventricle, resulting in a leftward shift of the end-diastolic pressure volume relationship toward normal values, which has been referred to . Publications All (17) WB (10) IHC (2) IF (6) See more citations 9, 658995. Molecular function for LAMP2 Gene according to UniProtKB/Swiss-Prot Function: Plays an important role in chaperone-mediated autophagy, a process that mediates lysosomal degradation of proteins in response to various stresses and as part of the normal turnover of proteins with a long biological half-live (PubMed: 8662539 , 11082038 , 18644871 . The molecular mechanism of miR221 in regulating TGF1induced HSC activation may provide novel insight into therapies to ameliorate the pathological progression of liver fibrosis. Gene Ontology Cellular Component. For proteins with a molecular weight in the range of 25 kDa, Mouse Anti-Rabbit IgG (Conformation Specific) (L27A9) mAb (or HRP conjugate #5127 . H4B4 has been reported to recognize all three LAMP-2 isoforms A, B, and C [PMID: 22365987], suggesting that the epitope is located on the luminal region of LAMP-2 common to all isoforms. The atomic weights of the elements found in organic substances are C = 12.011, H = 1.008, S = 32.065, O = 15.999, and N = 14.007. Cart Is Empty. However, recent . Calculated Molecular Weight (Da) 44961. GO Process (4) GO . The detection was neither a result of unspecific binding of the secondary antibody as seen in the right panel. Hepatocellular carcinoma (HCC), the most common type of liver cancer, is the leading cause of cancer-related mortality worldwide. Both are transmembrane proteins and are heavily glycosylated at the amino-terminal . LAMP 2 has a structure comprised of a large amino-terminal intra-lysosomal domain, a hydrophobic trans-membrane domain, and a short carboxyl-terminal cytoplasmic tail. LAMP2 was preferentially expressed by RPE cells, and its expression declined with age. 2021. MW molecular weight ladder. Furthermore, the positions of . . LAMP-2 molecular weight appears to be at ~105 kDa on the gel due to high glycosylation. Guidelines for the use and interpretation of assays for monitoring autophagy La Biblioteca Virtual en Salud es una coleccin de fuentes de informacin cientfica y tcnica en salud organizada y almacenada en formato electrnico en la Regin de Amrica Latina y el Caribe, accesible de forma universal en Internet de modo compatible con las bases internacionales. Mass spectrometry (MS) analysis showed that aldometanib blocked FBP by directly occupying the active centre of aldolase through conjugation to Lys230 (a critical residue that resides in the active centre of aldolase for FBP binding; see ref. LAMP-2 (Lysosome-associated membrane protein 2) is a single-pass type I membrane protein that belongs to a family of membrane glycoproteins (~40 KDa). Lysosomes are compartments in the cell that digest and recycle materials. The observed band in the RPE corresponded to mature, extensively glycosylated LAMP2, resulting in an apparent molecular weight of 100 kDa . LAMP2 was present in small vesicles with the N-terminal protein chain facing the lumen of the vesicle. Enzyme deficiency results in accumulation of partially degraded heparan sulfate (HS) within lysosomes throughout the body, leading . LAMP2 is a type I transmembrane protein consisting of a transmembrane domain, a small C-terminal cytoplasmic tail with a lysosomal membrane-targeting signal, and a large intraluminal domain.104 Danon cardiomyopathy. We conclude that lysophagic clearance of damaged lysosomes results in generation of. Lysosomal-associated membrane protein 1(LAMP-1) also known as lysosome-associated membrane glycoprotein 1and CD107a(Cluster of Differentiation 107a), is a proteinthat in humans is encoded by the LAMP1gene. Anti-LAMP2 Antibody [GL2A7] WB (1:1000), ICC/IF (1:500); optimal dilutions for assays should be determined by the user. Its protein, LAMP2, is one of the lysosome-associated membrane glycoproteins . Moreover, UA stabilized Parkin protein expression (Figure 4G). . Exogenous ceramide, a sphingolipid, has been well documented to exert anticancer effects. Astrocytes, the most numerous cells of the central nervous system, exert critical functions for brain homeostasis. Cart Is Empty. Biol. This glycoprotein provides selectins . Incubate the cells with complete DMEM or glucose-free DMEM. predicted N: Leu 29: SDS-PAGE: Purity > 85 % as determined by SDS-PAGE: Molecular Biotechnology and Gene Therapy, Paul-Ehrlich Institut, Langen, Germany. RRID: AB_2134767. Cell Reports. LAMP2B retains the lysosomal targeting signal, gly-tyr-x-x, and has the same number of potential glycosylation sites as LAMB2A. HOST SPECIES: Rabbit SPECIES REACTIVITY: Human IMMUNOGEN: This LAMP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 196-225 amino acids from the Central region of human LAMP2. Method 1 However, around 27% of sites display a relaxation of purifying constraints (average = 0.42128), among which, 14 particular sites undergo positive selection ( > 1). Lysosomal-associated membrane protein 2 (LAMP2, synonyms: LAMPB, CD107b) is a member of a family of membrane glycoproteins. Lysosome-associated membrane protein 2 ( LAMP2 ), also known as CD107b ( C luster of D ifferentiation 107b) and Mac-3, is a human gene.
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